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Progen Biotechnik aav elisa kits
Aav Elisa Kits, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav+elisa+kit/aav2+elisa+kit+titration/pm41297510-60-6-10
Average 86 stars, based on 1 article reviews
aav elisa kits - by Bioz Stars, 2026-10
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Related Articles

Virus:

Article Title: Local gene knockdown in the brain using viral-mediated RNA interference.
Article Snippet: Conditional mutant techniques that allow spatial and temporal control over gene expression can be used to create mice with restricted genetic modifications.. These mice serve as powerful disease models in which gene function in adult tissues can be specifically dissected.. Current strategies for conditional genetic manipulation are inefficient, however, and often lack sufficient spatial control.

Article Title: Modulation of GSK-3β/β-Catenin Signaling Contributes to Learning and Memory Impairment in a Rat Model of Depression
Article Snippet: .. The virus was then titered using an AAV ELISA kit (Progen) and stored at -80°C. ..

Article Title: Striatal overexpression of ΔJunD resets L-DOPA-induced dyskinesia in a primate model of Parkinson disease
Article Snippet: .. The virus was then titered using an AAV ELISA kit (Progen) and evaluated for infectivity in HT1080 cells. ..

Article Title: Protein Kinase C-Dependent Dephosphorylation of Tyrosine Hydroxylase Requires the B56δ Heterotrimeric Form of Protein Phosphatase 2A
Article Snippet: .. The virus was titered using an AAV ELISA kit (Progen) and kept at −80°C until time of use. ..

Article Title: Mania-like behavior induced by disruption of CLOCK
Article Snippet: .. The virus was then titered by using an AAV ELISA kit (Progen, Heidelberg, Germany) and used for infection of HT1080 cells. ..

Bioprocessing:

Article Title: Local gene knockdown in the brain using viral-mediated RNA interference.
Article Snippet: Conditional mutant techniques that allow spatial and temporal control over gene expression can be used to create mice with restricted genetic modifications.. These mice serve as powerful disease models in which gene function in adult tissues can be specifically dissected.. Current strategies for conditional genetic manipulation are inefficient, however, and often lack sufficient spatial control.

Article Title: Modulation of GSK-3β/β-Catenin Signaling Contributes to Learning and Memory Impairment in a Rat Model of Depression
Article Snippet: .. The virus was then titered using an AAV ELISA kit (Progen) and stored at -80°C. ..

Article Title: Striatal overexpression of ΔJunD resets L-DOPA-induced dyskinesia in a primate model of Parkinson disease
Article Snippet: .. The virus was then titered using an AAV ELISA kit (Progen) and evaluated for infectivity in HT1080 cells. ..

Article Title: Protein Kinase C-Dependent Dephosphorylation of Tyrosine Hydroxylase Requires the B56δ Heterotrimeric Form of Protein Phosphatase 2A
Article Snippet: .. The virus was titered using an AAV ELISA kit (Progen) and kept at −80°C until time of use. ..

Article Title: Mania-like behavior induced by disruption of CLOCK
Article Snippet: .. The virus was then titered by using an AAV ELISA kit (Progen, Heidelberg, Germany) and used for infection of HT1080 cells. ..

Enzyme-linked Immunosorbent Assay:

Article Title: Local gene knockdown in the brain using viral-mediated RNA interference.
Article Snippet: Conditional mutant techniques that allow spatial and temporal control over gene expression can be used to create mice with restricted genetic modifications.. These mice serve as powerful disease models in which gene function in adult tissues can be specifically dissected.. Current strategies for conditional genetic manipulation are inefficient, however, and often lack sufficient spatial control.

Article Title: Modulation of GSK-3β/β-Catenin Signaling Contributes to Learning and Memory Impairment in a Rat Model of Depression
Article Snippet: .. The virus was then titered using an AAV ELISA kit (Progen) and stored at -80°C. ..

Article Title: Striatal overexpression of ΔJunD resets L-DOPA-induced dyskinesia in a primate model of Parkinson disease
Article Snippet: .. The virus was then titered using an AAV ELISA kit (Progen) and evaluated for infectivity in HT1080 cells. ..

Article Title: Protein Kinase C-Dependent Dephosphorylation of Tyrosine Hydroxylase Requires the B56δ Heterotrimeric Form of Protein Phosphatase 2A
Article Snippet: .. The virus was titered using an AAV ELISA kit (Progen) and kept at −80°C until time of use. ..

Article Title: Mania-like behavior induced by disruption of CLOCK
Article Snippet: .. The virus was then titered by using an AAV ELISA kit (Progen, Heidelberg, Germany) and used for infection of HT1080 cells. ..

Infection:

Article Title: Local gene knockdown in the brain using viral-mediated RNA interference.
Article Snippet: Conditional mutant techniques that allow spatial and temporal control over gene expression can be used to create mice with restricted genetic modifications.. These mice serve as powerful disease models in which gene function in adult tissues can be specifically dissected.. Current strategies for conditional genetic manipulation are inefficient, however, and often lack sufficient spatial control.

Article Title: Striatal overexpression of ΔJunD resets L-DOPA-induced dyskinesia in a primate model of Parkinson disease
Article Snippet: .. The virus was then titered using an AAV ELISA kit (Progen) and evaluated for infectivity in HT1080 cells. ..

Article Title: Mania-like behavior induced by disruption of CLOCK
Article Snippet: .. The virus was then titered by using an AAV ELISA kit (Progen, Heidelberg, Germany) and used for infection of HT1080 cells. ..



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Analysis of genetic stability of rBV encoding rAAV genes through serial passages (A) Experimental layout of serial passage for rBV in Sf9 cells. Cell culture supernatant containing the budded rBV is subcultured into a new flask containing uninfected Sf9 cells every 72 h. (B–E) Quantification of baculovirus and <t>AAV</t> proteins. (B) and (D) Total budded BV and rBV were quantified from cell supernatant using ddPCR. (C) and (E) Capsid and Rep proteins were quantified from cell suspension using <t>ELISA.</t>
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Analysis of genetic stability of rBV encoding rAAV genes through serial passages (A) Experimental layout of serial passage for rBV in Sf9 cells. Cell culture supernatant containing the budded rBV is subcultured into a new flask containing uninfected Sf9 cells every 72 h. (B–E) Quantification of baculovirus and <t>AAV</t> proteins. (B) and (D) Total budded BV and rBV were quantified from cell supernatant using ddPCR. (C) and (E) Capsid and Rep proteins were quantified from cell suspension using <t>ELISA.</t>
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Analysis of genetic stability of rBV encoding rAAV genes through serial passages (A) Experimental layout of serial passage for rBV in Sf9 cells. Cell culture supernatant containing the budded rBV is subcultured into a new flask containing uninfected Sf9 cells every 72 h. (B–E) Quantification of baculovirus and <t>AAV</t> proteins. (B) and (D) Total budded BV and rBV were quantified from cell supernatant using ddPCR. (C) and (E) Capsid and Rep proteins were quantified from cell suspension using <t>ELISA.</t>
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Analysis of genetic stability of rBV encoding rAAV genes through serial passages (A) Experimental layout of serial passage for rBV in Sf9 cells. Cell culture supernatant containing the budded rBV is subcultured into a new flask containing uninfected Sf9 cells every 72 h. (B–E) Quantification of baculovirus and <t>AAV</t> proteins. (B) and (D) Total budded BV and rBV were quantified from cell supernatant using ddPCR. (C) and (E) Capsid and Rep proteins were quantified from cell suspension using <t>ELISA.</t>
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Analysis of genetic stability of rBV encoding rAAV genes through serial passages (A) Experimental layout of serial passage for rBV in Sf9 cells. Cell culture supernatant containing the budded rBV is subcultured into a new flask containing uninfected Sf9 cells every 72 h. (B–E) Quantification of baculovirus and <t>AAV</t> proteins. (B) and (D) Total budded BV and rBV were quantified from cell supernatant using ddPCR. (C) and (E) Capsid and Rep proteins were quantified from cell suspension using <t>ELISA.</t>
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Analysis of genetic stability of rBV encoding rAAV genes through serial passages (A) Experimental layout of serial passage for rBV in Sf9 cells. Cell culture supernatant containing the budded rBV is subcultured into a new flask containing uninfected Sf9 cells every 72 h. (B–E) Quantification of baculovirus and <t>AAV</t> proteins. (B) and (D) Total budded BV and rBV were quantified from cell supernatant using ddPCR. (C) and (E) Capsid and Rep proteins were quantified from cell suspension using <t>ELISA.</t>
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Analysis of Crude <t>AAV</t> samples using the AVR-NPR column (A) Chromatograms of the purified AAV8 (black), AAV8-mixed cell-cultured supernatant (purple), and AAV8-mixed cell-lysed supernatant (red). The gray polygonal line represents the ratio of mobile phase A (15 mM sodium acetate, 10 mM glycine, and 50 mM CaCl 2 [pH 4.5]) and B (15 mM sodium acetate, 10 mM glycine, and 50 mM CaCl 2 [pH 2.2]), where here the ratio of B increases linearly from 0% to 100% between 30 and 75 min after the start of the measurement. (B) Quantification of AAV8 using the AVR-NPR column and <t>ELISA.</t> The bars show the purified AAV8 (gray), AAV8-mixed cell-cultured supernatant (purple), and AAV8-mixed cell-lysed supernatant (red). The capsid titers quantified with each purified AAV8 are shown as 1 and the other capsid titers as relative values. The analyses were performed in triplicate, and the error bars indicate the standard deviation. (C–F) Expression monitoring of AAV8. Chromatograms of (C) cell-cultured and (D) cell-lysed supernatants expressing AAV8 at days 1, 2, 3, 4, and 7 after the transfection. The gray polygonal line represents the ratio of mobile phase A and B, where here the ratio of B increases linearly from 0% to 100% between 30 and 75 min after the start of the measurement. (E) Quantification of the AAV8 capsid titers for each sample in (C) and (D). The results of cell-cultured supernatants quantified by the AVR-NPR column and ELISA are plotted as gray squares with solid and dotted lines, respectively. In contrast, the results of cell-lysed supernatants quantified by the AVR-NPR column and ELISA are plotted as black circles with solid and dotted lines, respectively. (F) Correlation functions between the AAV8 capsid titers quantified by the AVR-NPR column and ELISA. Gray squares and black circles were plotted based on the quantification results of cell-cultured and cell-lysed supernatant in (E), respectively. Each dotted line indicates the linear trendline calculated from the plots. R-squared values of the trendlines are shown in the graph.
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Values of loss (orange, calculated as the ratio of the <t>AAV</t> titer in the flow-through and wash fractions vs. load) and yield (green, calculated as the ratio of the AAV titer in the elution fraction vs. load) of (A) AAV2 and (B) AAV9 obtained via bind-and-elute studies in non-competitive mode using peptide-based resins KFNHWFG- (W1), WKAHNKG- (W2), IWWHIAKFG- (W3), FWNWHHFKG- (W4), FWWAAFFKG- (W5), IAFKKISIG- (W6), IKIFFFFSG- (W7), KWWIWAG- (W8), WWIKISG- (W9), FFNFFKG- (W10), FNHFFIG- (W11), GYISRHPG- (W12) Toyopearl resins, and control adsorbents POROS™ CaptureSelect™ AAVX and AVB Sepharose HP resins. The AAV titer in the flow-through, wash, and elution fractions was measured using serotype-specific <t>ELISA</t> kits.
Aav Titration Elisa Kit, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Analysis of genetic stability of rBV encoding rAAV genes through serial passages (A) Experimental layout of serial passage for rBV in Sf9 cells. Cell culture supernatant containing the budded rBV is subcultured into a new flask containing uninfected Sf9 cells every 72 h. (B–E) Quantification of baculovirus and AAV proteins. (B) and (D) Total budded BV and rBV were quantified from cell supernatant using ddPCR. (C) and (E) Capsid and Rep proteins were quantified from cell suspension using ELISA.

Journal: Molecular Therapy Advances

Article Title: Continuous production of recombinant adeno-associated virus in the insect cell/baculovirus expression vector system

doi: 10.1016/j.omta.2026.201744

Figure Lengend Snippet: Analysis of genetic stability of rBV encoding rAAV genes through serial passages (A) Experimental layout of serial passage for rBV in Sf9 cells. Cell culture supernatant containing the budded rBV is subcultured into a new flask containing uninfected Sf9 cells every 72 h. (B–E) Quantification of baculovirus and AAV proteins. (B) and (D) Total budded BV and rBV were quantified from cell supernatant using ddPCR. (C) and (E) Capsid and Rep proteins were quantified from cell suspension using ELISA.

Article Snippet: Cell lysates were used for the quantification of total Rep proteins using AAV Rep ELISA kit (Cell Biolabs, California, USA) according to the manufacturer’s protocol.

Techniques: Cell Culture, Suspension, Enzyme-linked Immunosorbent Assay

Continuous rAAV production using a three-tank cascade (A) Total BVs and rBVs were quantified from cell culture supernatant using ddPCR with primers to target the lef2 and AAV genes (B) rAAV genome titer was quantified from cell suspension quantified using ddPCR. (C) Quantification of total AAV capsid produced during the continuous production from cell suspension (D) Rep concentration from cell lysates using ELISA (E) SYPRO Ruby staining of denatured AAV capsids produced from the continuous production showing viral proteins (VPs). (F) Infectivity of budded rBV stored in spent media for 3 months at 4°C. The budded rBVs collected at different time points were used to infect Sf9 cells to produce rAAV.

Journal: Molecular Therapy Advances

Article Title: Continuous production of recombinant adeno-associated virus in the insect cell/baculovirus expression vector system

doi: 10.1016/j.omta.2026.201744

Figure Lengend Snippet: Continuous rAAV production using a three-tank cascade (A) Total BVs and rBVs were quantified from cell culture supernatant using ddPCR with primers to target the lef2 and AAV genes (B) rAAV genome titer was quantified from cell suspension quantified using ddPCR. (C) Quantification of total AAV capsid produced during the continuous production from cell suspension (D) Rep concentration from cell lysates using ELISA (E) SYPRO Ruby staining of denatured AAV capsids produced from the continuous production showing viral proteins (VPs). (F) Infectivity of budded rBV stored in spent media for 3 months at 4°C. The budded rBVs collected at different time points were used to infect Sf9 cells to produce rAAV.

Article Snippet: Cell lysates were used for the quantification of total Rep proteins using AAV Rep ELISA kit (Cell Biolabs, California, USA) according to the manufacturer’s protocol.

Techniques: Cell Culture, Suspension, Produced, Concentration Assay, Enzyme-linked Immunosorbent Assay, Staining, Infection

Analysis of Crude AAV samples using the AVR-NPR column (A) Chromatograms of the purified AAV8 (black), AAV8-mixed cell-cultured supernatant (purple), and AAV8-mixed cell-lysed supernatant (red). The gray polygonal line represents the ratio of mobile phase A (15 mM sodium acetate, 10 mM glycine, and 50 mM CaCl 2 [pH 4.5]) and B (15 mM sodium acetate, 10 mM glycine, and 50 mM CaCl 2 [pH 2.2]), where here the ratio of B increases linearly from 0% to 100% between 30 and 75 min after the start of the measurement. (B) Quantification of AAV8 using the AVR-NPR column and ELISA. The bars show the purified AAV8 (gray), AAV8-mixed cell-cultured supernatant (purple), and AAV8-mixed cell-lysed supernatant (red). The capsid titers quantified with each purified AAV8 are shown as 1 and the other capsid titers as relative values. The analyses were performed in triplicate, and the error bars indicate the standard deviation. (C–F) Expression monitoring of AAV8. Chromatograms of (C) cell-cultured and (D) cell-lysed supernatants expressing AAV8 at days 1, 2, 3, 4, and 7 after the transfection. The gray polygonal line represents the ratio of mobile phase A and B, where here the ratio of B increases linearly from 0% to 100% between 30 and 75 min after the start of the measurement. (E) Quantification of the AAV8 capsid titers for each sample in (C) and (D). The results of cell-cultured supernatants quantified by the AVR-NPR column and ELISA are plotted as gray squares with solid and dotted lines, respectively. In contrast, the results of cell-lysed supernatants quantified by the AVR-NPR column and ELISA are plotted as black circles with solid and dotted lines, respectively. (F) Correlation functions between the AAV8 capsid titers quantified by the AVR-NPR column and ELISA. Gray squares and black circles were plotted based on the quantification results of cell-cultured and cell-lysed supernatant in (E), respectively. Each dotted line indicates the linear trendline calculated from the plots. R-squared values of the trendlines are shown in the graph.

Journal: Molecular Therapy. Methods & Clinical Development

Article Title: Engineering a highly durable adeno-associated virus receptor for analytical applications

doi: 10.1016/j.omtm.2023.101157

Figure Lengend Snippet: Analysis of Crude AAV samples using the AVR-NPR column (A) Chromatograms of the purified AAV8 (black), AAV8-mixed cell-cultured supernatant (purple), and AAV8-mixed cell-lysed supernatant (red). The gray polygonal line represents the ratio of mobile phase A (15 mM sodium acetate, 10 mM glycine, and 50 mM CaCl 2 [pH 4.5]) and B (15 mM sodium acetate, 10 mM glycine, and 50 mM CaCl 2 [pH 2.2]), where here the ratio of B increases linearly from 0% to 100% between 30 and 75 min after the start of the measurement. (B) Quantification of AAV8 using the AVR-NPR column and ELISA. The bars show the purified AAV8 (gray), AAV8-mixed cell-cultured supernatant (purple), and AAV8-mixed cell-lysed supernatant (red). The capsid titers quantified with each purified AAV8 are shown as 1 and the other capsid titers as relative values. The analyses were performed in triplicate, and the error bars indicate the standard deviation. (C–F) Expression monitoring of AAV8. Chromatograms of (C) cell-cultured and (D) cell-lysed supernatants expressing AAV8 at days 1, 2, 3, 4, and 7 after the transfection. The gray polygonal line represents the ratio of mobile phase A and B, where here the ratio of B increases linearly from 0% to 100% between 30 and 75 min after the start of the measurement. (E) Quantification of the AAV8 capsid titers for each sample in (C) and (D). The results of cell-cultured supernatants quantified by the AVR-NPR column and ELISA are plotted as gray squares with solid and dotted lines, respectively. In contrast, the results of cell-lysed supernatants quantified by the AVR-NPR column and ELISA are plotted as black circles with solid and dotted lines, respectively. (F) Correlation functions between the AAV8 capsid titers quantified by the AVR-NPR column and ELISA. Gray squares and black circles were plotted based on the quantification results of cell-cultured and cell-lysed supernatant in (E), respectively. Each dotted line indicates the linear trendline calculated from the plots. R-squared values of the trendlines are shown in the graph.

Article Snippet: The AAV Titration ELISA Kit series (PROGEN Biotechnik, Heidelberg, Germany) was used to quantify AAV capsid titers depending on the AAV serotype.

Techniques: Purification, Cell Culture, Enzyme-linked Immunosorbent Assay, Standard Deviation, Expressing, Transfection

Values of loss (orange, calculated as the ratio of the AAV titer in the flow-through and wash fractions vs. load) and yield (green, calculated as the ratio of the AAV titer in the elution fraction vs. load) of (A) AAV2 and (B) AAV9 obtained via bind-and-elute studies in non-competitive mode using peptide-based resins KFNHWFG- (W1), WKAHNKG- (W2), IWWHIAKFG- (W3), FWNWHHFKG- (W4), FWWAAFFKG- (W5), IAFKKISIG- (W6), IKIFFFFSG- (W7), KWWIWAG- (W8), WWIKISG- (W9), FFNFFKG- (W10), FNHFFIG- (W11), GYISRHPG- (W12) Toyopearl resins, and control adsorbents POROS™ CaptureSelect™ AAVX and AVB Sepharose HP resins. The AAV titer in the flow-through, wash, and elution fractions was measured using serotype-specific ELISA kits.

Journal: Biotechnology and bioengineering

Article Title: Peptide ligands for the affinity purification of adeno-associated viruses from HEK 293 cell lysates

doi: 10.1002/bit.28495

Figure Lengend Snippet: Values of loss (orange, calculated as the ratio of the AAV titer in the flow-through and wash fractions vs. load) and yield (green, calculated as the ratio of the AAV titer in the elution fraction vs. load) of (A) AAV2 and (B) AAV9 obtained via bind-and-elute studies in non-competitive mode using peptide-based resins KFNHWFG- (W1), WKAHNKG- (W2), IWWHIAKFG- (W3), FWNWHHFKG- (W4), FWWAAFFKG- (W5), IAFKKISIG- (W6), IKIFFFFSG- (W7), KWWIWAG- (W8), WWIKISG- (W9), FFNFFKG- (W10), FNHFFIG- (W11), GYISRHPG- (W12) Toyopearl resins, and control adsorbents POROS™ CaptureSelect™ AAVX and AVB Sepharose HP resins. The AAV titer in the flow-through, wash, and elution fractions was measured using serotype-specific ELISA kits.

Article Snippet: The titer of AAV particles in the feedstock, flow-through, and elution fractions collected as described in Section 2.5 and Section 2.6 was measured using a AAV Titration ELISA kit (PROGEN, Wayne, PA) following the manufacturer’s protocol.

Techniques: Control, Enzyme-linked Immunosorbent Assay

Values of loss (orange, calculated as the ratio of the AAV titer in the flow-through and wash fractions vs. load) and yield (green, calculated as the ratio of the AAV titer in the elution fraction vs. load) of AAV2 and logarithmic reduction of HCPs (HCP LRV, red triangles) obtained via chromatographic purification of AAV2 from a clarified HEK 293 cell lysate (AAV2 titer: ~1.6·1011 vp/mL; HCP titer: ~0.5 mg/mL) using (A) control adsorbents POROS™ CaptureSelect™ AAVX and AVB Sepharose HP resins as well as peptide-based resins KFNHWFG- (W1), WKAHNKG- (W2), IWWHIAKFG- (W3), FWNWHHFKG- (W4), FWWAAFFKG- (W5), (B) IAFKKISIG- (W6), IKIFFFFSG- (W7), KWWIWAG- (W8), WWIKISG- (W9), FFNFFKG- (W10), FNHFFIG- (W11), GYISRHPG- (W12) Toyopearl resins. The AAV titer in the flow-through, wash, and elution fractions was measured using serotype-specific ELISA kits.

Journal: Biotechnology and bioengineering

Article Title: Peptide ligands for the affinity purification of adeno-associated viruses from HEK 293 cell lysates

doi: 10.1002/bit.28495

Figure Lengend Snippet: Values of loss (orange, calculated as the ratio of the AAV titer in the flow-through and wash fractions vs. load) and yield (green, calculated as the ratio of the AAV titer in the elution fraction vs. load) of AAV2 and logarithmic reduction of HCPs (HCP LRV, red triangles) obtained via chromatographic purification of AAV2 from a clarified HEK 293 cell lysate (AAV2 titer: ~1.6·1011 vp/mL; HCP titer: ~0.5 mg/mL) using (A) control adsorbents POROS™ CaptureSelect™ AAVX and AVB Sepharose HP resins as well as peptide-based resins KFNHWFG- (W1), WKAHNKG- (W2), IWWHIAKFG- (W3), FWNWHHFKG- (W4), FWWAAFFKG- (W5), (B) IAFKKISIG- (W6), IKIFFFFSG- (W7), KWWIWAG- (W8), WWIKISG- (W9), FFNFFKG- (W10), FNHFFIG- (W11), GYISRHPG- (W12) Toyopearl resins. The AAV titer in the flow-through, wash, and elution fractions was measured using serotype-specific ELISA kits.

Article Snippet: The titer of AAV particles in the feedstock, flow-through, and elution fractions collected as described in Section 2.5 and Section 2.6 was measured using a AAV Titration ELISA kit (PROGEN, Wayne, PA) following the manufacturer’s protocol.

Techniques: Purification, Control, Enzyme-linked Immunosorbent Assay